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| Chatelier, Aurelien; Imbert, Nathalie; Zambonino, Jose-luis; Mckenzie, David; Bois, P. |
| The present study employed a patch clamp technique in isolated seabass ventricular myocytes to investigate the hypothesis that oleic acid (OA), a mono-unsaturated fatty acid, can exert direct effects upon whole-cell barium currents. Acute application of free OA caused a dose-dependent depression of the whole-cell barium current that was evoked by a voltage step to 0 mV from a holding potential of -80 mV. The derived 50% inhibitory concentration (IC50) was 12.49 +/- 0.27 mu mol l(-1). At a concentration of 30 mu mol l(-1), OA significantly reduced the current density to about 45% of control values, but did not modify either the shape of the current-density voltage relationship or the apparent reversal potential. In addition, OA did not modify the voltage... |
| Tipo: Text |
Palavras-chave: Fatty acid; Sea bass; Ventricular myocyte; L type calcium channel; Oleic acid. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2120.pdf |
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| Claireaux, Guy; Desaunay, Yves; Akcha, Farida; Auperin, Benoît; Bocquene, Gilles; Budzinski, Hélène; Cravedi, Jean-pierre; Davoodi, Fariba; Galois, Robert; Gilliers, Camille; Goanvec, Christelle; Guerault, Daniel; Imbert, Nathalie; Mazeas, Olivier; Nonnotte, Guy; Nonnotte, Liliane; Prunet, Patrick; Sebert, Philippe; Vettier, Aurélie. |
| Evaluating the ecological impact of an oil spill is a complex issue requiring coherently articulated examination of the sequence of interactions that link the cell, where contaminants exert their effects, to the ecosystem, where interactions with human activities arise. This sequence of interactions traverses the frontiers between scientific disciplines (chemistry, toxicology, physiology, and fisheries ecology). Using the common sole (Solea solea L.) as a model species for the coastal habitats polluted by the "Erika" oil spill, our research project attempted to define indices of functional integrity that characterised the consequences of fuel exposure at the different biological levels. The coupling of field observations with experimental laboratory work... |
| Tipo: Text |
Palavras-chave: Physiological ecology; Common sole; Complex system approach; Oil spill. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-405.pdf |
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